jurkat cell viability  (R&D Systems)

Journal: Technology in Cancer Research & Treatment

Article Title: Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways

doi: 10.1177/1533033820947489

Figure Lengend Snippet: Effect of Thymoquinone and/or DFMO on Jurkat cell viability. Cells were exposed to increasing concentrations of DFMO (A) or TQ (B) for 24 h. To evaluate the synergistic effect on cell viability, cells were treated with either DFMO (1 mM) for 48 h or TQ (10 μM) or incubated with 1 mM of DFMO for 24 h before adding 10 μM of TQ for additional 24 h (C). Cell viability rate was assessed by WST-1 assay, as indicated in the methods and materials. The data are representative of 3 different experiments. Values are shown as means ± S.E.M. (n = 3); *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.0001, ## , p < 0.01, && , p < 0.01 versus respective control.

Article Snippet: Jurkat cell viability rate was also determined by cell counting using the trypan blue exclusion method (Invitrogen).

Techniques: Incubation, WST-1 Assay, Control

Journal: Technology in Cancer Research & Treatment

Article Title: Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways

doi: 10.1177/1533033820947489

Figure Lengend Snippet: DFMO and Thymoquinone synergize to induce apoptosis in Jurkat cells. To evaluate the synergistic effect on apoptosis, cells were treated with either DFMO at 1 mM for 48 h or TQ at 10 μM or incubated with 1 mM of DFMO for 24 h before adding TQ at (10 μM) for additional 24 h (A & B). To confirm the synergistic effect of TQ and DFMO, cells were treated with either DFMO at 0.5 mM for 48 h or TQ at 20 μM or incubated with 0.5 mM of DFMO for 24 h before adding TQ at (20 μM) for additional 24 h. Apoptosis in Jurkat cells was assessed by flow cytometry using the Annexin V/7AAD staining apoptosis assay (A, B, C & D). Values are shown as means ± S.E.M. (n = 3); *, p < 0.05, ***, p < 0.001, ****, p < 0.0001, ### , p < 0.001, #### , p < 0.0001, && , p < 0.01, &&&& , p < 0.0001 versus respective control.

Article Snippet: Jurkat cell viability rate was also determined by cell counting using the trypan blue exclusion method (Invitrogen).

Techniques: Incubation, Flow Cytometry, Staining, Apoptosis Assay, Control

Journal: Technology in Cancer Research & Treatment

Article Title: Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways

doi: 10.1177/1533033820947489

Figure Lengend Snippet: Downregulated Genes Triggered in DFMO-Treated Jurkat Cells as Compared with Untreated Cells.

Article Snippet: Jurkat cell viability rate was also determined by cell counting using the trypan blue exclusion method (Invitrogen).

Techniques: Gene Expression, Ubiquitin Proteomics, Histone Deacetylase Assay

Journal: Technology in Cancer Research & Treatment

Article Title: Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways

doi: 10.1177/1533033820947489

Figure Lengend Snippet: Upregulated Tumor Suppressor Genes in DFMO-Treated Jurkat Cells as Compared With Untreated Cells.

Article Snippet: Jurkat cell viability rate was also determined by cell counting using the trypan blue exclusion method (Invitrogen).

Techniques:

Journal: Technology in Cancer Research & Treatment

Article Title: Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways

doi: 10.1177/1533033820947489

Figure Lengend Snippet: Upregulated Pro-Apoptotic Genes in DFMO-Treated Jurkat Cells as Compared With Untreated Cells.

Article Snippet: Jurkat cell viability rate was also determined by cell counting using the trypan blue exclusion method (Invitrogen).

Techniques:

Journal: Technology in Cancer Research & Treatment

Article Title: Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways

doi: 10.1177/1533033820947489

Figure Lengend Snippet: Heat map of the deregulated genes in treated versus control cells. The signature of the deregulated genes are represented in the intensity of color; with the alteration of LogFC (fold change) from -1 to +3 in DFMO-treated Jurkat cells as compared to the untreated cells.

Article Snippet: Jurkat cell viability rate was also determined by cell counting using the trypan blue exclusion method (Invitrogen).

Techniques: Control

Journal: Technology in Cancer Research & Treatment

Article Title: Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways

doi: 10.1177/1533033820947489

Figure Lengend Snippet: Synergistic effect of TQ and DFMO on the expression of UHRF1, DNMT1 and HDAC1 mRNA levels in Jurkat cells. To evaluate the synergistic effect on the expression of UHRF1, DNMT1 and HDAC1 genes, cells were treated with either DFMO (1 mM) for 48 h or TQ (10 μM) or incubated with 1 mM of DFMO for 24 h before adding 10 μM of TQ for additional 24 h. The histograms show the quantification data of mRNA expressions of UHRF1 (A), DNMT1 (B) and HDAC1 (C), as assessed by real-time PCR. Results are means of 3 separate experiments performed in triplicate. Values are shown as means ± S.E.M. (n = 3); *, p < 0.05, ***, p < 0.001, ****, p < 0.0001, ## , p < 0.01, ### , p < 0.001, &&& , p < 0.001, ### , p < 0.001, &&&& , p < 0.0001 versus respective control.

Article Snippet: Jurkat cell viability rate was also determined by cell counting using the trypan blue exclusion method (Invitrogen).

Techniques: Expressing, Incubation, Real-time Polymerase Chain Reaction, Control

Journal: Technology in Cancer Research & Treatment

Article Title: Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways

doi: 10.1177/1533033820947489

Figure Lengend Snippet: Effect of the depletion of UHRF1 cell viability. Jurkat cells were transfected with siRNA against UHRF1 for 72 h. (A): Western blot was then performed using an anti-UHRF1 antibody as described in materials and methods. (B): Cell viability was calculated using trypan blue as indicated in materials and methods. Data are shown as mean ± SE of 3 independent experiments ( #### P < 0.0001, ***P < 0.001 versus respective control).

Article Snippet: Jurkat cell viability rate was also determined by cell counting using the trypan blue exclusion method (Invitrogen).

Techniques: Transfection, Western Blot, Control

Journal: British Journal of Cancer

Article Title: Prostaglandin E 2 stimulates Fas ligand expression via the EP1 receptor in colon cancer cells

doi: 10.1038/sj.bjc.6604490

Figure Lengend Snippet: Reduction in Jurkat T-cell viability following co-culture with colon cancer cells treated with PGE 2 . HT29 and HCA-7 cells were serum-deprived for 48 h, followed by treatment for 24 h with 0.1% DMSO, 0.5 or 1.0 μ M PGE 2 . Cells were fixed and then co-cultured with Jurkat T cells for 24 h. Jurkat T-cell viability was determined by resazurin reduction. To block FasL-mediated apoptosis, Jurkat T cells were also co-cultured with 1.0 μ M PGE 2 -treated colon cancer cells in the presence of 10 μ g ml −1 recombinant Fas:Fc blocking protein. Data denote mean±s.e.m. and are representative of two independent experiments. Viability is expressed as an index (% of control Jurkat T cells maintained in medium only).

Article Snippet: After 24 h, Jurkat cell viability was assessed by measuring the increase in fluorescence intensity associated with the cellular reduction of resazurin to resorufin, according to the manufacturer's instructions (R&D Systems).

Techniques: Co-Culture Assay, Cell Culture, Blocking Assay, Recombinant, Control