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jurkat cell viability  (R&D Systems)


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    Structured Review

    R&D Systems jurkat cell viability
    Reduction in <t>Jurkat</t> <t>T-cell</t> <t>viability</t> following co-culture with colon cancer cells treated with PGE 2 . HT29 and HCA-7 cells were serum-deprived for 48 h, followed by treatment for 24 h with 0.1% DMSO, 0.5 or 1.0 μ M PGE 2 . Cells were fixed and then co-cultured with Jurkat T cells for 24 h. Jurkat T-cell viability was determined by resazurin reduction. To block FasL-mediated apoptosis, Jurkat T cells were also co-cultured with 1.0 μ M PGE 2 -treated colon cancer cells in the presence of 10 μ g ml −1 recombinant Fas:Fc blocking protein. Data denote mean±s.e.m. and are representative of two independent experiments. Viability is expressed as an index (% of control Jurkat T cells maintained in medium only).
    Jurkat Cell Viability, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 225 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jurkat cell viability/product/R&D Systems
    Average 94 stars, based on 225 article reviews
    jurkat cell viability - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Prostaglandin E 2 stimulates Fas ligand expression via the EP1 receptor in colon cancer cells"

    Article Title: Prostaglandin E 2 stimulates Fas ligand expression via the EP1 receptor in colon cancer cells

    Journal: British Journal of Cancer

    doi: 10.1038/sj.bjc.6604490

    Reduction in Jurkat T-cell viability following co-culture with colon cancer cells treated with PGE 2 . HT29 and HCA-7 cells were serum-deprived for 48 h, followed by treatment for 24 h with 0.1% DMSO, 0.5 or 1.0 μ M PGE 2 . Cells were fixed and then co-cultured with Jurkat T cells for 24 h. Jurkat T-cell viability was determined by resazurin reduction. To block FasL-mediated apoptosis, Jurkat T cells were also co-cultured with 1.0 μ M PGE 2 -treated colon cancer cells in the presence of 10 μ g ml −1 recombinant Fas:Fc blocking protein. Data denote mean±s.e.m. and are representative of two independent experiments. Viability is expressed as an index (% of control Jurkat T cells maintained in medium only).
    Figure Legend Snippet: Reduction in Jurkat T-cell viability following co-culture with colon cancer cells treated with PGE 2 . HT29 and HCA-7 cells were serum-deprived for 48 h, followed by treatment for 24 h with 0.1% DMSO, 0.5 or 1.0 μ M PGE 2 . Cells were fixed and then co-cultured with Jurkat T cells for 24 h. Jurkat T-cell viability was determined by resazurin reduction. To block FasL-mediated apoptosis, Jurkat T cells were also co-cultured with 1.0 μ M PGE 2 -treated colon cancer cells in the presence of 10 μ g ml −1 recombinant Fas:Fc blocking protein. Data denote mean±s.e.m. and are representative of two independent experiments. Viability is expressed as an index (% of control Jurkat T cells maintained in medium only).

    Techniques Used: Co-Culture Assay, Cell Culture, Blocking Assay, Recombinant



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    R&D Systems jurkat cell viability
    Reduction in <t>Jurkat</t> <t>T-cell</t> <t>viability</t> following co-culture with colon cancer cells treated with PGE 2 . HT29 and HCA-7 cells were serum-deprived for 48 h, followed by treatment for 24 h with 0.1% DMSO, 0.5 or 1.0 μ M PGE 2 . Cells were fixed and then co-cultured with Jurkat T cells for 24 h. Jurkat T-cell viability was determined by resazurin reduction. To block FasL-mediated apoptosis, Jurkat T cells were also co-cultured with 1.0 μ M PGE 2 -treated colon cancer cells in the presence of 10 μ g ml −1 recombinant Fas:Fc blocking protein. Data denote mean±s.e.m. and are representative of two independent experiments. Viability is expressed as an index (% of control Jurkat T cells maintained in medium only).
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    Image Search Results


    Reduction in Jurkat T-cell viability following co-culture with colon cancer cells treated with PGE 2 . HT29 and HCA-7 cells were serum-deprived for 48 h, followed by treatment for 24 h with 0.1% DMSO, 0.5 or 1.0 μ M PGE 2 . Cells were fixed and then co-cultured with Jurkat T cells for 24 h. Jurkat T-cell viability was determined by resazurin reduction. To block FasL-mediated apoptosis, Jurkat T cells were also co-cultured with 1.0 μ M PGE 2 -treated colon cancer cells in the presence of 10 μ g ml −1 recombinant Fas:Fc blocking protein. Data denote mean±s.e.m. and are representative of two independent experiments. Viability is expressed as an index (% of control Jurkat T cells maintained in medium only).

    Journal: British Journal of Cancer

    Article Title: Prostaglandin E 2 stimulates Fas ligand expression via the EP1 receptor in colon cancer cells

    doi: 10.1038/sj.bjc.6604490

    Figure Lengend Snippet: Reduction in Jurkat T-cell viability following co-culture with colon cancer cells treated with PGE 2 . HT29 and HCA-7 cells were serum-deprived for 48 h, followed by treatment for 24 h with 0.1% DMSO, 0.5 or 1.0 μ M PGE 2 . Cells were fixed and then co-cultured with Jurkat T cells for 24 h. Jurkat T-cell viability was determined by resazurin reduction. To block FasL-mediated apoptosis, Jurkat T cells were also co-cultured with 1.0 μ M PGE 2 -treated colon cancer cells in the presence of 10 μ g ml −1 recombinant Fas:Fc blocking protein. Data denote mean±s.e.m. and are representative of two independent experiments. Viability is expressed as an index (% of control Jurkat T cells maintained in medium only).

    Article Snippet: After 24 h, Jurkat cell viability was assessed by measuring the increase in fluorescence intensity associated with the cellular reduction of resazurin to resorufin, according to the manufacturer's instructions (R&D Systems).

    Techniques: Co-Culture Assay, Cell Culture, Blocking Assay, Recombinant